This method or protocol describes nuclear and cytoplasmic fractionation of tissue culture cells and a method for Western blot detection of proteins using the Odyssey Infrared Imaging System. This method or protocol was used to detect expression of the "small" Tap protein in 293T, HeLa and COS cells. The Odyssey system has several advantages over the more widely used chemiluminescent detection methods.

Protocol: Western Blot Analysis of Sub-Cellular Fractionated Samples Using the Odyssey Infrared Imaging System