This method or protocol is designed to detect sequences in the murine genome by polymerase chain reaction amplification, and is adapted from Stratman and Simon (Transgenic Res. 12, 521-522 (2003)).For those familiar with PCR genotyping, this method differs from the typical method or protocol by utilizing a unique enzyme (Klentaq), 30mer primers, and a 68° annealing temperature.

Protocol: Universal Mouse Genotyping Protocol