This procedure, which uses a root transformation method or protocol, provides a rapid method for assessing gene expression in Arabidopsis roots. It is useful for testing promoter:reporter gene constructs, for expressing genes, the overexpression of which is lethal in whole plants, and for transforming the roots of plants that are recalcitrant to conventional transformation techniques. The method or protocol has been used successfully with Ws, No-0, and RLD ecotypes.

Protocol: Transgene Expression in Regenerated Roots