Protocol: Transformation of E. Coli by Electroporation Protocol.
Protocol: Transformation of E. Coli by Electroporation Protocol.
Protocol: Transformation of E. Coli by Electroporation Protocol.
<p>Electrocompetent bacteria are prepared by growing cultures to<sup> </sup>mid-log phase, washing the bacteria extensively at low temperature,<sup> </sup>and then resuspending them in a solution of low ionic strength<sup> </sup>containing glycerol. DNA is introduced during exposure of the<sup> </sup>bacteria to a short high-voltage electrical discharge.</p>
Protocol: Transformation of E. Coli by Electroporation Protocol.
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