The method, protocol describes a technique for the delivery of siRNAs into mammalian cells in the absence of reporter plasmids. This is best achieved with transfection reagents developed for the delivery of antisense oligodeoxynucleotides. The quantities of reagents given below are calculated for the transfection of one well of a 24-well plate.

Protocol: Transfection of Mammalian Cells with siRNA Duplexes Protocol