The following procedure is for simultaneous transfection and plating of RAW 264.7 cells. This method or protocol results in approximately 50% to 70% cell viability, and of those viable cells, 20% to 40% are transfected when using pEYFP-N1 from Clontech. Include: Procedure for Splitting Cells before Transfection; Procedure for Preparing Lipofectamine 2000 and DNA; Preparation of RAW 264.7 Cells for Transfection.

Protocol: Transfecting and Plating RAW 264.7 Cells with Lipofectamine 2000 Protocol