Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomics. Although many methods exist for fractionating proteins, the method described here can capture the majority of subcellular fractions simultaneously at reasonable purity. The scalability of this method makes it amenable to small samples, such as embryonic tissues, in addition to larger tissues. The method or protocol described is for the general fractionation and extraction of proteins from organs / tissue

Protocol: Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomic