Sophisticated fluorescence microscopy methods & equipment, now allow cellular events to be studied at high resolution in living material. The studying of living fly tissues presents unique difficulties in keeping the cells alive, introducing fluorescent probes, & imaging through thick hazy cytoplasm. This method or protocol outlines the preparation of major tissue types amenable to study by time-lapse cinematography and different methods for keeping them alive.

Protocol: Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material