The cell-attached capacitance recording technique is a powerful technique that has been successfully used to resolve single vesicle fusion and fusion pore conductance.  This technique, however, has not been applied to synapses because of the difficulty in accessing release sites.  Here, we developed a technique to expose release sites in a large nerve terminal, the calyx of Held, which contains clear-core glutamatergic vesicles.

Protocol: The Cell Attached Capacitance Recording Technique