Protocol: Tetracycline as Regulator of Inducible Gene Expression Protocol II
Protocol: Tetracycline as Regulator of Inducible Gene Expression Protocol II
Protocol: Tetracycline as Regulator of Inducible Gene Expression Protocol II
This stage of the procedure describes the transfection with target genes of cell lines already expressing inducible tTA. In this example, the target genes are transfected on a plasmid that carries puromycin resistance as a selectable marker.
Protocol: Tetracycline as Regulator of Inducible Gene Expression Protocol II
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