The method, protocol describes a technique to stain nerve fibers in tissue slices of avian embryos using an antibody against the 160-kD subunit of neurofilaments.  This allows the comparison of the branching pattern of motor and sensory neurons between control and experimental embryos.  The tissue is cut in slices using a vibratome or tissue slicer.  The method or protocol is suitable for older embryos after approximately stage 33 and regions that are not accessible by whole-mount analysis.

Protocol: Staining of Tissue Slices for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos