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Protocol: siRNA Double Transfection of RAW 264.7 Cells with Lipofectamine Protocol

Protocol: siRNA Double Transfection of RAW 264.7 Cells with Lipofectamine Protocol

Protocol: siRNA Double Transfection of RAW 264.7 Cells with Lipofectamine Protocol

Protocol Involves the transfection of siRNA into RAW 264. 7 cells using Lipofectamine 2000.  Cells are transfected with siRNA twice (on subsequent days).  Target gene knockdown is assessed from total RNA isolated 48 hr post-transfection or from protein isolated 72 hr post-transfection.

Protocol: siRNA Double Transfection of RAW 264.7 Cells with Lipofectamine Protocol

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