Protocol: Ribocloning: DNA Cloning and Gene Construction Using PCR Primers Terminated with a Ribonucleotide
Protocol: Ribocloning: DNA Cloning and Gene Construction Using PCR Primers Terminated with a Ribonucleotide
Protocol: Ribocloning: DNA Cloning and Gene Construction Using PCR Primers Terminated with a Ribonucleotide
Protocol exploits the discovery that Rnase A can efficiently cleave at single rC or rU bases embedded in double-stranded DNA. Entire plasmid vectors are amplified using long, high-fidelity PCR with riboprimers, which carry a single rC residue at their 3' end. Target DNA is amplified using similar primers, which also end in a rC residue.
Find more Protocols: PCR Cloning: Protocols: PCR CloningOverall Rating: (0 votes). Please rate this product now!
