Protocol: Quantification of Apoptosis and Cell Cycle Distribution of Primary B Cells Using Propidium Iodide
Protocol: Quantification of Apoptosis and Cell Cycle Distribution of Primary B Cells Using Propidium Iodide
Protocol: Quantification of Apoptosis and Cell Cycle Distribution of Primary B Cells Using Propidium Iodide
The water soluble, DNA intercalator, propidium iodide (PI), is used to bind to DNA after permeabilization of cells with NP40. The amount of dye bound correlates with the content of DNA within a given cell. Once cells are stained, they are analyzed on a flow cytometer. The relative content of DNA indicates the distribution of a population of cells throughout the cell cycle.
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