Although Percoll gradients were able to provide a purified sporocyst fraction, because these particles do not all band in a discrete manner in such gradients, they were unable to provide a simultaneous isolation of a pure oocyst wall fraction. Gradients formed from this method or protocol on the other hand are able to provide purified sporocysts and oocyst walls in the same gradient.

Protocol: Purification of Oocyst Walls and Sporocysts from Toxoplasma gondii Protocol