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Protocol: Purification of Lin, ckit+, Sca1+ Bone Marrow Cells for Culture, Flow Cytometry, or Transplantaio

Protocol: Purification of Lin, ckit+, Sca1+ Bone Marrow Cells for Culture, Flow Cytometry, or Transplantaio

Protocol: Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells for Culture, Flow Cytometry, or Transplantaio

Protocol for purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion. Includes: Antibodies for Lineage Depletion; Harvest Marrow; Lineage Depletion; Cell Sorting; Viral Transduction; Transplant; CFU assays; GFP and cell surface marker immunostaining.

Protocol: Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells for Culture, Flow Cytometry, or Transplantaio

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