Protocol: Purification of HistidineTagged Proteins Using IMAC Without Parameter Optimization Protocol
Protocol: Purification of HistidineTagged Proteins Using IMAC Without Parameter Optimization Protocol
Protocol: Purification of Histidine-Tagged Proteins Using IMAC Without Parameter Optimization Protocol
Histidine-tagged proteins can be purified on prepacked 1-ml immobilized metal-ion affinity chromatography (IMAC) columns without optimization of the separation conditions. The method allows fast capture of the target protein, although with a lower purity than can be obtained under optimized conditions.
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