Protocol: Purification of Fusion Proteins by Affinity Chromatography on Glutathione Agarose Protocol

Recombinant proteins, constructed in pGEX vectors, are fused to glutathione S-transferase (GST) and can be purified to near homogeneity by affinity chromatography on glutathione-agarose.  Bound GST-fusion proteins are readily displaced from the column by elution with buffers containing free glutathione.

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Protocol: Purification of Fusion Proteins by Affinity Chromatography on Glutathione Agarose Protocol