The method, protocol describes a technique of the acquisition of confocal fluorescent and bright field images of live cells, expressing cyan fluorescent protein (CFP) and/or yellow fluorescent protein (YFP), with a spinning disk confocal head on a Zeiss Axiovert 200 M microscope. Protocol includes: Description of Microscope and Imaging Setup; Description of Acquisition Parameters; Image Processing; Movie Processing.

Protocol: Protocol Describing Live Cell Spinning Disk Confocal Fluorescence Imaging of Cells on a Zeiss