T cell hybridomas can be obtained by fusing activated T cells with tumor cells. A heterogeneous population of hybridomas can be cloned by limiting dilution to obtain hybridomas that express specificity to one T cell receptor (TCR). The method, protocol describes a technique of cell fusion and selection of T cell hybridomas. A method or protocol is provided for screening of T cell hybridomas for expression of the CD3-TCR complex by flow cytometry analysis.

Protocol: Production of Mouse T Cell Hybridomas Protocol