Protocol: Preparation of siRNAs from Drosophila Embryo Extracts Protocol

siRNAs produced upon the addition of dsRNA to Drosophila embryo extract are enriched in a micrococcus-nuclease-resistant fraction.  After proteinase K treatment and dephosphorylation with calf intestinal phosphatase, these siRNAs mediate efficient RNAi in vitro.

Find more Protocols: RNAi Drosophila Protocols: Protocols: RNAi Drosophila Protocols

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Protocol: Preparation of siRNAs from Drosophila Embryo Extracts Protocol