The method or protocol provides a method to achieve a sample sufficient for one determination of cAMP using the acetylation method or protocol. The method or protocol describes the method used for measuring the content of cyclic adenosine 3',5'- monophosphate (cyclic AMP or cAMP) in RAW 264.7 cells using an enzyme-linked immunoassay system. Information included in the method or protocol: Treatment of Cells and Preparation of Extracts; Reagents and Materials.

Protocol: Preparation of RAW 264.7 Lysates for Cyclic AMP Determination