Protocol: Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage {lambda} Lysogens
Protocol: Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage {lambda} Lysogens
Protocol: Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage {lambda} Lysogens
In this method or protocol, a bacterial lysogen is constructed from a recombinant bacteriophage {lambda} encoding a fusion protein of interest. The resulting lysogenic colonies are induced to synthesize the fusion protein, which is then isolated in preparation for functional and biochemical analyses.
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