This method or protocol provides a sufficient sample for several determinations of cAMP using the acetylation method or protocol. The method chosen for measuring the content of cyclic adenosine 3',5'-monophosphate (cyclic AMP or cAMP) in splenic B lymphocytes (B cells) is an enzyme linked immunoassay system. Protocol includes information on: Treatment of Cells and Preparation of Extracts; Reagents and Materials.

Protocol: Preparation of B-Lymphocyte Lysates for Cyclic AMP Determination