The method, protocol describes a technique of a procedure for adapting conventional HPLC systems to provide accurate low-flow rates (0.4-4 µl/min) and gradients required to operate slurry-packed capillary columns.  A key component of this system is a commercial axial-beam longitudinal flow cell that can be fitted to a number of commercial UV detectors.

Protocol: Packing Capillary Columns for RP-HPLC Protocol