Protocol: Optimization of Imidazole Concentrations for Immobilized MetalIon Affinity Chromatography Protocol
Protocol: Optimization of Imidazole Concentrations for Immobilized MetalIon Affinity Chromatography Protocol
Protocol: Optimization of Imidazole Concentrations for Immobilized Metal-Ion Affinity Chromatography Protocol
Protocol for the optimization of imidazole concentrations for immobilized metal-ion affinity chromatography. Most samples from which histidine-tagged proteins are to be purified also contain endogenous protein contaminants that bind to the immobilized metal-ion affinity chromatography (IMAC) adsorbent. Usually, these proteins bind more weakly than the histidine-tagged protein.
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