Protocol: Mutation Detection by SolidPhase Chemical Cleavage of Mismatches Using Radioactive Probes Protocol
Protocol: Mutation Detection by SolidPhase Chemical Cleavage of Mismatches Using Radioactive Probes Protocol
Protocol: Mutation Detection by Solid-Phase Chemical Cleavage of Mismatches Using Radioactive Probes Protocol
This method or protocol is the first of two describing solid-phase chemical cleavage of mismatch (SpCCM), a method for the detection of mutations in genomic DNA. DNA fragments up to 2 kb in length can be analyzed to determine the position (within 10-15 bp) and identity of the mismatched nucleotide.
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