The more commonly available single-laser cytometers can also be used to measure multicellular conjugates, but due to overlaps in emission spectra, the extent of labeling cells with fluorophores must be controlled much more carefully when the single-laser machines are used. This method or protocol describes the labeling of cells and analysis of conjugates with either dual-laser or single laser flow cytometers.

Protocol: Measurement of Intercellular Conjugates by Flow Cytometry Protocol