The most commonly used markers for selection of transgenic Arabidopsis are resistance to the antibiotic kanamycin and to the herbicide glufosinate ammonium. Resistance to kanamycin is conferred by a bacterial gene encoding the enzyme neomycin phosphotransferase (NPT). In this method or protocol, kanamycin-resistant seedlings are selected on solid medium.

Protocol: Kanamycin Selection of Transformed Arabidopsis Protocol