Protocol for the isolation of Arabidopsis nuclei and measurement of gene transcription rates using nuclear run-on assays. Plant materials are ground in hexylene glycol-based buffers and highly enriched nuclear fractions are obtained using Percoll density gradients. Standard and small-scale method or protocols are presented, along with a tested method for nuclear run-on assays. The entire process may be completed within 3 days.

Protocol: Isolation of Arabidopsis Nuclei and Measurement of Gene Transcription Rates Protocol