Protocol: Immunoblotting: SemiDry Electrophoretic Transfer of Proteins from Gels to Membranes Protocol
Protocol: Immunoblotting: SemiDry Electrophoretic Transfer of Proteins from Gels to Membranes Protocol
Protocol: Immunoblotting: Semi-Dry Electrophoretic Transfer of Proteins from Gels to Membranes Protocol
The transfer of proteins from a Tris/glycine SDS-polyacrylamide gel to a membrane using a semi-dry method is achieved by placing the gel next to a piece of nitrocellulose filter. This sandwich is placed directly between two plate electrodes, and the proteins are then transferred from the gel onto the filter.
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