Protocol: Gentle lysis of mammalian cells for cytochrome c release assay

Lysis in the sucrose-containing Buffer ("Mito-Buffer") is supposed to prevent accidential disrupture of the mitochondria to prevent the leakage of mitochondrial proteins (such as cytochrome c) into the cytosol. Celldeath.de

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Protocol: Gentle lysis of mammalian cells for cytochrome c release assay