Protocol: Generation of Bidirectional Sets of Deletion Mutants by Digestion with BAL 31 Nuclease Protocol
Protocol: Generation of Bidirectional Sets of Deletion Mutants by Digestion with BAL 31 Nuclease Protocol
Protocol: Generation of Bidirectional Sets of Deletion Mutants by Digestion with BAL 31 Nuclease Protocol
In this method, the nuclease BAL 31 is used to make uni- or bidirectional deletions in a segment of cloned DNA. BAL 31 is a complex enzyme and tends to digest a population of double-stranded DNA targets in an asynchronous fashion, Deletions created by BAL 31 are therefore far more heterogeneous in size than those created by processive enzymes such as exonuclease III.
Find more Protocols: Mutation Detection: Protocols: Mutation DetectionOverall Rating: (0 votes). Please rate this product now!
