Protocol: Freezing and Thawing of Embryonic Stem (ES) Cells Using Cryovials Protocol

This method or protocol describes a method for freezing and thawing ES cells using cryovials. It is important to freeze ES cell stocks as soon as possible to reduce the time that they are in culture. A careful record should be kept of the number of times cells are passaged and the location of the cryovials.

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Protocol: Freezing and Thawing of Embryonic Stem (ES) Cells Using Cryovials Protocol