Protocol: Flow Cytometric Analysis of Mitochondrial Transmembrane Potential ({Delta}{Psi}m)

The method, protocol describes a technique for evaluation of mitochondrial function using the fluorochrome CMXRos. CMXRos is sequestered by actively respiring mitochondria, but washed out when the mitochondrial membrane potential is lost. This analysis can be combined with the TUNEL technique or immunocytochemistry.

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Protocol: Flow Cytometric Analysis of Mitochondrial Transmembrane Potential ({Delta}{Psi}m)