The goal of this method is to identify transcriptionally active genes in cloned segments of genomic DNA. The method or protocol uses hybridization and affinity purification to recover biotin-labeled cDNAs that bind to a 500-kb segment of human DNA cloned in a BAC vector. However, the method can be easily adapted to other clones of genomic DNAs cloned in high-capacity vectors.

Protocol: Direct Selection of cDNAs with Large Genomic DNA Clones Protocol