Pluripotent ES cells can develop into many types of differentiated tissues if they are placed back into a differentiating environment. Often, differentiation proceeds through an intermediate stage called the embryoid body (EB). EBs can be manipulated further to generate more differentiated cell types. This method or protocol describes a method for differentiation of ES cells into EBs.

Protocol: Differentiating Embryonic Stem (ES) Cells into Embryoid Bodies Protocol