Protocol: Cell Staining for Sorting of Hematopoietic Stem Cells and Myeloid Progenitors and Isolating RNA
Protocol: Cell Staining for Sorting of Hematopoietic Stem Cells and Myeloid Progenitors and Isolating RNA
Protocol: Cell Staining for Sorting of Hematopoietic Stem Cells and Myeloid Progenitors and Isolating RNA
The combination of prospective identification/isolation of bone marrow progenitors and quantitative RT-PCR is a powerful tool to understand the molecular mechanism underlying hematopoiesis. Here, we described our standard procedures of the murine myeloid progenitor staining for fluorescence activated cells sorting (FACS) and RNA purification methods.
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