This method or protocol describes an in vitro transcription assay that allows for a single round of transcription from in vitro assembled chromatin. Comparing the activity of a receptor or transcriptional coactivator in an assay that measures only a single round of transcription with the results from multiple rounds of transcription can help elucidate the mechanism of transcriptional activation by those factors.

Protocol: Assay:Single Round of In Vitro Transcription from Assembled Chromatin Templates Using a HeLa Cell Ex