Protocol: Annealing, Cloning, and Sequencing of shRNA Linkers into pEN_H1 Plasmids Protocol
Protocol: Annealing, Cloning, and Sequencing of shRNA Linkers into pEN_H1 Plasmids Protocol
Protocol: Annealing, Cloning, and Sequencing of shRNA Linkers into pEN_H1 Plasmids Protocol
The method, protocol describes a technique of the preparation of an shRNA-expressing Gateway entry vector. This process is preceded by the design and synthesis of target gene-specific sense and antisense oligonucleotides which, when annealed, will constitute an shRNA cassette.
Protocol: Annealing, Cloning, and Sequencing of shRNA Linkers into pEN_H1 Plasmids Protocol
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