Protocol: An Alternatively Spliced Isoform of t(8;21) Transcript Promotes Leukemogenesis
Protocol: An Alternatively Spliced Isoform of t(8;21) Transcript Promotes Leukemogenesis
Protocol: An Alternatively Spliced Isoform of t(8;21) Transcript Promotes Leukemogenesis
Describe the methods to identify and quantitate the specific A/E9a transcript in t(8;21) patients samples relative to the AML1-ETO transcript encoding the well known full-length 752 amino acid AML1-ETO protein (AE). Includes: RNA preparation and RT-PCR; Relative quantitation of the AE9a and the AE transcripts.
Protocol: An Alternatively Spliced Isoform of t(8;21) Transcript Promotes Leukemogenesis
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